Split homing CRISPR in Anopheles stephensi for research and development - Pirbright Institute

Asian malaria mosquito

Intended use

The aim is to 'pave the way for future gene drive designs with multiple sgRNAs expressed by multiple RNA Pol III promoters to mitigate resistance.'

Readiness of Technology

5 - Limited proof of concept in laboratory

Project leader

Alphey L

Institution

Pirbright Institute, UK

Publications

Authors name for system

CRISPR-Cas9 based gene drive

Class of Technology

Split homing gene drive

Field(s) of application

General research and development

Description of system

Split homing CRISPR targeting cardinal gene - four variants with different promoters for the sgRNA

Research in target organism

✔

Research in model organism

✖

Intended for release

✖

Intended direct effects

NA - Proof of concept