Split homing CRISPR gene drive in Fusarium graminearum - CSIRO Queensland

Intended use

To modify populations of F. graminearum to disrupt virulence factors in this species, and so reduce head blight in wheat and barley

Readiness of Technology

4a - Active research on gene drive construction

Project leader

Kazan K

Institution

CSIRO, Queensland, Australia

Project leader

Gardiner DM

Institution

CSIRO, Queensland, Australia

Publications

Gardiner et al., 2020

Authors name for system

Do not use any particular term – but give detailed description of design

Class of Technology

Split homing gene drive

Field(s) of application

Agriculture

Description of system

Split homing CRISPR gene drive

For specialists

Notes on readiness of technology

The authors state 'When a strain expressing Cas9 and a TRI5 targeting guide RNA (DTRI465Cas9-sgRNA::TRI5) was used in place of the Spok1 insert in the TRI465 locus (Gardiner & Kazan, 2018) equal segregation (1 : 1, n = 16) was observed, suggesting strongly that this particular nuclease-based design, similar to high-efficiency drive systems shown to work in a number of other species including yeast and insects (DiCarlo et al., 2015; Hammond et al., 2016), is unable to drive in F. graminearum.'

This design is therefore designated as non-functional.

Research in target organism

✔

Research in model organism

✖

Intended for release

✖

Intended direct effects

Population modification/replacement