Preliminary work towards a homing CRISPR gene drive in Vespula germanica - University of Otago

German wasp

Intended use

Reduction of ecological effects of invasive populations of this species in New Zealand and elsewhere (e.g. Australia)

Readiness of Technology

2a - Gene drive proposed with supporting theoretical work

Project leader

Dearden PK

Institution

University of Otago, New Zealand

Publications

Authors name for system

NA – preliminary theoretical study

Field(s) of application

Agriculture

Conservation

Description of system

Homing CRISPR

Description

Homing CRISPR targeting a spermatogenesis gene. Possible targets include: boule1, ocnus, and sdic genes.

Notes on readiness of technology

Modelling study suggesting feasibility of targeting this haplodiploid species with a suppression gene drive targeting male fertility.

+ Tested CRISPR- Cas cleavage of possible target genes in vitro.

+ Genome sequencing undertaken.

Phenotype

Partially infertile males

Research in target organism

✖

Research in model organism

✖

Intended for release

✖

Intended direct effects

Population suppression/eradication